Carbon-Ion Beam Radiosensitivity Study and Biological Responses of High-Yielding Rice Line, MR219-PL-5

The carbon ion-beam has emerged as a novel physical mutagen for creating genetic variability and crop improvement. In this study, seeds of high-yielding pyramided rice line MR219-PL-5 were exposed to ion beam irradiation at 10, 20, 40, 60, 80, 100 Gy. radiosensitivity test was conducted determine the optimum dose based on lethal 50% (LD50) using Sandwich Blotter Technique. biological responses carbon-ion also observed in other characteristics such germination rate (GeR), survival (SR), growth (GRoR), shoot length (SL), root (RL), seedling height (SH), days flowering (DTF), fertility (FR) thousand-grains weight (TGW). Based polynomial curve SR graph, value 86.12 However, range between 40 60 Gy these two doses recorded highest SR, 63 67%, respectively. Furthermore, shoulder study since decreased significantly higher doses. M1 individuals irradiated had best where significant differences found SL, RL, GRoR, SH, DTF FR compared Further studies M2 M3 populations could help identify potential well understand inheritance each trait interest from one generation next.